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Indian Mustard [Brassica juncea (L.) Czern.]

  • Protocol
Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 343))

Summary

All economically important Brassica species have been successfully transformed using Agrobacterium tumefaciens. Although different tissues have been used as explants, hypocotyls remain the most desirable explants for Brassica tissue culture owing to their amenability to regeneration. Young explants excised from 3- to 4-d-old seedlings have exhibited optimal regeneration potential; the addition of adjuvants such as silver nitrate to the selection medium is necessary to achieve high efficiency of transformation. This chapter describes an Agrobacterium-mediated transformation protocol for Indian mustard based on inoculation of hypocotyls. The selectable marker gene used encodes for neomycin phosphotransferase II (nptII), and the selection agent is kanamycin.

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Authors
  1. Ksenija Gasic
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  2. Schuyler S. Korban
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Editor information

Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, and Department of Agronomy, Iowa State University, Ames, Iowa, USA

    Kan Wang

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© 2006 Humana Press Inc.

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Gasic, K., Korban, S.S. (2006). Indian Mustard [Brassica juncea (L.) Czern.]. In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 343. Humana Press. https://doi.org/10.1385/1-59745-130-4:281

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  • DOI: https://doi.org/10.1385/1-59745-130-4:281

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-536-1

  • Online ISBN: 978-1-59745-130-7

  • eBook Packages: Springer Protocols

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