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Agrobacterium Protocols pp 185–200Cite as

Maize (Zea mays L.)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 343))

Summary

Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.

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Authors
  1. Bronwyn R. Frame
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  2. Tina Paque
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  3. Kan Wang
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Editor information

Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, and Department of Agronomy, Iowa State University, Ames, Iowa, USA

    Kan Wang

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© 2006 Humana Press Inc.

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Cite this protocol

Frame, B.R., Paque, T., Wang, K. (2006). Maize (Zea mays L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 343. Humana Press. https://doi.org/10.1385/1-59745-130-4:185

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  • DOI: https://doi.org/10.1385/1-59745-130-4:185

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-536-1

  • Online ISBN: 978-1-59745-130-7

  • eBook Packages: Springer Protocols

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