Peas (Pisum sativum L.)

  • Jan Grant
  • Pauline Cooper
Part of the Methods in Molecular Biology book series (MIMB, volume 343)

Summary

In this chapter we describe a robust method for transformation of peas that has been successfully used in our laboratory since 1992. Using immature pea seed collected from field- or greenhouse-grown plants, we have produced transgenic lines for over 30 genotypes including named pea cultivars and advanced breeding lines. This method uses immature cotyledons as the explant, and the transformation efficiency is in the range 0.2 to 13.5% of cotyledons producing at least one independently transformed line. Agrobacterium tumefaciens strains AGL1 and KYRT1 are the most successful in our procedure, and kanamycin, phosphinothricin, and hygromycin are reliable selectable markers. Potentially useful genes have been introduced for pest and disease resistance, altering quality traits, and investigating metabolic pathways and are being studied in transgenic pea lines.

Key Words

Peas Pisum sativum transformation immature cotyledons legumes Agrobacterium tumefaciens transgenic plants 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Jan Grant
  • Pauline Cooper

There are no affiliations available

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