Abstract
Small interefence RNA (siRNA) has revamped the technology of gene silencing in cultured mammalian cells after its first demonstration by Tushl et al. 4 yr ago. To circumvent the cost and the inconvenience in identifying a unique siRNA duplex that can quench target gene expression, we devised a reporter-based system to test knockdown efficiency of selected siRNAs. We demonstrated that this luciferase-based siRNA testing system can be used to evaluate the knockdown efficiency of a directly transfected siRNA duplex or an siRNA expressed from a lentiviral vector.
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Zhuang, F., Liu, YH. (2006). Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology™, vol 342. Humana Press. https://doi.org/10.1385/1-59745-123-1:181
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DOI: https://doi.org/10.1385/1-59745-123-1:181
Publisher Name: Humana Press
Print ISBN: 978-1-58829-581-1
Online ISBN: 978-1-59745-123-9
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