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Cell-Cell Interactions pp 251–281Cite as

Isolation and Analysis of Lipid Rafts in Cell–Cell Interactions

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 341))

Abstract

Lipid rafts are dynamic structures made up of proteins and lipids that float freely within the liquid-disordered bilayer of cellular membranes and have the ability to cluster to form larger, more-ordered platforms. These clustered structures have been identified in all cell types and have been shown to play critical roles in signal transduction, cellular transport, and Cell-Cell communication. Lipid rafts also have been implicated in facilitating bacterial/viral entry into host cells and in human disease, highlighting the significance of understanding the role lipid rafts play in physiological and pathological signaling outcomes. In this chapter, we provide protocols to isolate lipid rafts from polarized and nonpolarized cells and outline novel technologies to analyze signal transduction cascades in vivo.

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Author information

Authors and Affiliations

  1. Department of Molecular Biology, Massachusetts General Hospital, Boston, MA

    Aimee Landry & Ramnik Xavier

Authors
  1. Aimee Landry
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  2. Ramnik Xavier
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Editor information

Editors and Affiliations

  1. Center for Experimental Therapeutics and Reperfusion Injury, Brigham

    Sean P. Colgan

  2. Women’s Hospital, Havard Medical School, Boston, MA, USA

    Sean P. Colgan

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© 2006 Humana Press Inc.

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Landry, A., Xavier, R. (2006). Isolation and Analysis of Lipid Rafts in Cell–Cell Interactions. In: Colgan, S.P. (eds) Cell-Cell Interactions. Methods in Molecular Biology™, vol 341. Humana Press. https://doi.org/10.1385/1-59745-113-4:251

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  • DOI: https://doi.org/10.1385/1-59745-113-4:251

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-523-1

  • Online ISBN: 978-1-59745-113-0

  • eBook Packages: Springer Protocols

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