Construction of microRNA-Containing Vectors for Expression in Mammalian Cells

  • Yoko Fukuda
  • Hiroaki Kawasaki
  • Kazunari Taira
Part of the Methods in Molecular Biology book series (MIMB, volume 338)


MicroRNAs (miRNAs) are a class of noncoding RNAs that regulate gene expression by single-stranded RNAs of 18 to 25 nucleotides in length. Hundreds of miRNAs have been found in animals and plants, some of which play important roles in development or differentiation. Increasing attention has thus been paid to their biogenesis and regulation mechanisms and the identification of target genes. We are constructing a comprehensive expression vector library containing predicted human miRNAs. miRNA expression vectors containing human RNA polymerase II or III promoters, and utilizing a flexible vector system, can be useful for functional analysis.

Key Words

MicroRNA CMV promoter U6 promoter 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Yoko Fukuda
    • 1
  • Hiroaki Kawasaki
    • 1
  • Kazunari Taira
    • 1
  1. 1.Department of Chemistry and BiotechnologyThe University of TokyoJapan

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