Abstract
Nasopharyngeal carcinoma (NPC) is one of the most common cancers in Southern China. Epstein-Barr virus (EBV) infection is an important etiological factor of NPC. The fact that EBV genome is present in almost all NPC tissues renders it an ideal tumor marker for NPC. To date, quantitative analysis of plasma EBV DNA has been shown to be clinically useful for the detection, monitoring, and prognostication of NPC. In addition, the molecular nature of circulating EBV DNA has recently been identified as that of free DNA fragments; it is not contained inside intact virions. By quantitative size analysis, it is further demonstrated that more than 80% of these DNA fragments are less than 180 bp in size. In this chapter, the clinical applications of plasma EBV DNA analysis and the protocols for the quantitative analysis of the size of circulating EBV DNA will be discussed.
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Acknowledgments
This work was supported by an Earmarked Research Grant (CUHK 4276/ 04M) from the Research Grants Council of the Hong Kong Special Administrative Region(China).
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Chan, K.C.A., Lo, Y.M.D. (2006). Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative Analysis of the Size of Circulating Epstein-Barr Virus DNA. In: Lo, Y.M.D., Chiu, R.W.K., Chan, K.C.A. (eds) Clinical Applications of PCR. Methods in Molecular Biology™, vol 336. Humana Press. https://doi.org/10.1385/1-59745-074-X:111
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DOI: https://doi.org/10.1385/1-59745-074-X:111
Publisher Name: Humana Press
Print ISBN: 978-1-58829-348-0
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