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In Vitro Assays for Immune Monitoring in Transplantation

  • Protocol
Transplantation Immunology

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 333))

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Abstract

Because immune responses to transplant allografts are the main drivers of rejection, the ability to accurately quantitate antidonor immunity is an important goal in clinical transplantation. These allow for the prediction of presensitization to the transplanted tissue and the identification of rejection without needing more invasive tests.

In this chapter, we will review three methods currently used in transplantation research. Limiting dilution assays are a traditional tool. The evolution of these assays has brought about the ELISpot. Developments in flow cytometry are also contributing to the understanding of the composition of the cells involved in these immune responses.

We can therefore obtain a deeper understanding of the process of rejection and tolerance and their evolution with time. This chapter reviews in vitro assays in the context of transplantation, but the scientific applications of sensitive, accurate, and specific immunemonitoring reach well beyond this field of research.

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Author information

Authors and Affiliations

  1. Immunoregulation Laboratory, Department of Nephrology and Transplantation, School of Medicine, Kings College London, University of London, London, England

    Maria P. Hernandez-Fuentes

  2. Renal Section, Division of Medicine, Hammersmith Hospital, Imperial College London, London, England

    Alan Salama

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  1. Maria P. Hernandez-Fuentes
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  2. Alan Salama
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Editors and Affiliations

  1. National Heart and Lung Institute, London, UK

    Philip Hornick

  2. National Heart and Lung Institute, Harefield, UK

    Marlene Rose

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© 2006 Humana Press Inc.

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Hernandez-Fuentes, M.P., Salama, A. (2006). In Vitro Assays for Immune Monitoring in Transplantation. In: Hornick, P., Rose, M. (eds) Transplantation Immunology. Methods In Molecular Biology™, vol 333. Humana Press. https://doi.org/10.1385/1-59745-049-9:269

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  • DOI: https://doi.org/10.1385/1-59745-049-9:269

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-544-6

  • Online ISBN: 978-1-59745-049-2

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