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Real-Time Quantitative Polymerase Chain Reaction in Cardiac Transplant Research

  • Leanne E. Felkin
  • Anne B. Taegtmeyer
  • Paul J. R. Barton
Part of the Methods In Molecular Biology™ book series (MIMB, volume 333)

Abstract

The real-time quantitative polymerase chain reaction (PCR), an increasingly popular technique for the detection of DNA, combines a high degree of accuracy with extreme sensitivity. In this chapter we describe the use of real-time quantitative PCR in trans-plantation research in two areas in which this method is commonly applied: the accurate quantification of mRNA in tissue samples and genotyping of DNA. These are described in the context of cardiac transplantation, but they are of equal relevance to other areas of transplant biology.

Key Words

Real-time PCR quantitative PCR mRNA quantification TaqMan RiboGreen RNA degradation genotyping single-nucleotide polymorphism (SNP) polymorphism myocardial biopsy 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Leanne E. Felkin
    • 1
  • Anne B. Taegtmeyer
    • 1
  • Paul J. R. Barton
    • 1
  1. 1.National Heart and Lung Institute, Imperial College London, Heart Science CentreHarefield HospitalHarefieldEngland

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