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Human Embryonic Stem Cell Protocols pp 43–53Cite as

Derivation and Maintenance of Human Embryonic Stem Cells

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Part of the book series: Methods In Molecular Biology ((MIMB,volume 331))

Abstract

Since their derivation in 1998, human embryonic stem cells (hESCs) have been the center of tremendous scientific efforts in improve the existing methodologies for their isolation and maintenance to exhaust the potential use of these unique cells in cell-based therapy and developmental research. To date, there are more than 50 reported well-characterized hESC lines worldwide. hESCs are traditionally isolated from the blastocysts on mouse embryonic fibroblasts. The most used method for isolating the inner cell mass from the human blastocyst is immunosurgery. This chapter focuses on the basic methods for the derivation and maintenance of hESC lines.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Rambam Medical Centre The Faculty of Medicine, Technion, Haifa, Israel

    Michal Amit & Joseph Itskovitz-Eldor

Authors
  1. Michal Amit
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  2. Joseph Itskovitz-Eldor
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Editor information

Editors and Affiliations

  1. Ottawa Health Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2006 Humana Press Inc.

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Amit, M., Itskovitz-Eldor, J. (2006). Derivation and Maintenance of Human Embryonic Stem Cells. In: Turksen, K. (eds) Human Embryonic Stem Cell Protocols. Methods In Molecular Biology, vol 331. Humana Press. https://doi.org/10.1385/1-59745-046-4:43

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  • DOI: https://doi.org/10.1385/1-59745-046-4:43

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-497-5

  • Online ISBN: 978-1-59745-046-1

  • eBook Packages: Springer Protocols

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