Abstract
Dopaminergic neurons of human origin have many potential research applications such as in vitro studies on biochemical pathways related to neuronal disorders, and potentially direct cell replacement for therapeutic use. Dopaminergic neurons with apparently normal properties can be produced from embryonic stem cells from mice and sub-human primates by the simple procedure of coculturing with the PA6 stromal cell line. Recently, we have demonstrated that this coculture system can induce dopaminergic differentiation in human embryonic stem cells, and the human embryonic stem cell-derived dopaminergic cells exhibit biochemical and functional properties of mature dopaminergic neurons.
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References
Thomson J. A., Itskovitz-Eldor J., Shapiro S. S., et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147.
Lee S. H., Lumelsky N., Studer L., Auerbach J. M., and McKay R. D. (2000) Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nat. Biotechnol. 18, 675–679.
Kim J. H., Auerbach J. M., Rodriguez-Gomez J. A., et al. (2002) Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson’s disease. Nature 418, 50–56.
Kawasaki H., Mizuseki K., Nishikawa S., et al. (2000) Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron 28, 31–40.
Morizane A., Takahashi J., Takagi Y., Sasai Y., and Hashimoto N. (2002) Optimal conditions for in vivo induction of dopaminergic neurons from embryonic stem cells through stromal cell-derived inducing activity. J. Neurosci. Res. 69,934–939.
Zeng X., Chen J., Sanchez J. F., et al. (2003) Stable expression of hrGFP by mouse embryonic stem cells: promoter activity in the undifferentiated state and during dopaminergic neural differentiation. Stem Cells 21, 647–653.
Kawasaki H., Suemori H., Mizuseki K., et al. (2002) Generation of dopaminergicneurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity. Proc. Natl. Acad. Sci. USA 99, 1580–1585.
Zeng X, Miura T., Luo Y., et al. (2004) Properties of pluripotent human embryonicstem cells BG01 and BG02. Stem Cells 22, 292–312.
Zeng X., Cai J., Chen J., et al. (2004) Dopaminergic differentiation of human embryonic stem cells. Stem Cells 22, 925–940.
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© 2006 Humana Press Inc.
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Carpenter, M., Rao, M.S., Freed, W.J., Zeng, X. (2006). Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic Stem Cells In Vitro. In: Turksen, K. (eds) Human Embryonic Stem Cell Protocols. Methods In Molecular Biology, vol 331. Humana Press. https://doi.org/10.1385/1-59745-046-4:153
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DOI: https://doi.org/10.1385/1-59745-046-4:153
Publisher Name: Humana Press
Print ISBN: 978-1-58829-497-5
Online ISBN: 978-1-59745-046-1
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