Massively Parallel Signature Sequencing

  • Daixing Zhou
  • Mahendra S. Rao
  • Roger Walker
  • Irina Khrebtukova
  • Christian D. Haudenschild
  • Takumi Miura
  • Shannon Decola
  • Eric Vermaas
  • Keith Moon
  • Thomas J. Vasicek
Part of the Methods In Molecular Biology book series (MIMB, volume 331)

Abstract

Massively parallel signature sequencing is an ultra-high throughput sequencing technology. It can simultaneously sequence millions of sequence tags, and, therefore, is ideal for whole genome analysis. When applied to expression profiling, it reveals almost every transcript in the sample and provides its accurate expression level. This chapter describes the technology and its application in establishing stem cell transcriptome databases.

Key Words

Massively parallel signature sequencing gene expression stem cell transcriptome 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Daixing Zhou
    • 1
  • Mahendra S. Rao
    • 2
  • Roger Walker
    • 1
  • Irina Khrebtukova
    • 1
  • Christian D. Haudenschild
    • 1
  • Takumi Miura
    • 1
  • Shannon Decola
    • 1
  • Eric Vermaas
    • 3
  • Keith Moon
    • 1
  • Thomas J. Vasicek
    • 1
  1. 1.Lynx Therapeutics Inc.Hayward
  2. 2.Stem Cell Biology Unit, Laboratory of NeurosciencesNational Institute on AgingBaltimore
  3. 3.Department of Research and DevelopmentSolexa Inc.Hayward

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