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Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens

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Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 329))

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Abstract

The process of bringing a new pharmacologically active drug to market is laborious, time consuming, and costly. From drug discovery to safety assessment, new methods are constantly sought to develop faster and more efficient procedures to eliminate drugs from further investigation because of their limited effectiveness or high toxicity. Because in vitro cell assays are an important arm of this discovery process, it is therefore somewhat unsurprising that there is an emerging contribution of embryonic stem (ES) cell technology to this area. This technology utilizes the in vitro differentiation of ES cells into somatic cell target populations that, when coupled to the use of “lineage selection” protocols, allows for the production of infinite numbers of pure populations of the desired cells for both bioactivity and toxicological screens. Unlike the use of transformed cell lines, ES-derived cells remain karyotypically normal and therefore better reflect the potential responses of cells in vivo, and when selected are more homogeneous than those obtained using primary cultures. In this chapter we discuss the use of ES cell-derived somatic cells in pharmacological screens, with particular emphasis on neural cells, and describe the methods and protocols associated with the development of ES cell-derived neural cell assays.

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Author information

Authors and Affiliations

  1. Stem Cell Sciences Ltd., University of Edinburgh, Edinburgh, UK

    Patrick J. Mee

  2. Stem Cell Sciences Ltd., Melbourne, Victoria, Australia

    Carmel M. O’Brien

  3. Stem Cell Sciences Ltd., University of Edinburgh, Edinburgh, UK

    Hazel Thomson

  4. Stem Cell Sciences Ltd., University of Edinburgh, Edinburgh, UK

    Sjaak van der Sar

  5. Eli Lilly and Company Ltd., Erl Wood Manor, Surrey, UK

    Viktor Lakics

  6. Stem Cell Sciences Ltd., University of Edinburgh, Edinburgh, UK

    Timothy E. Allsopp

Authors
  1. Patrick J. Mee
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  2. Carmel M. O’Brien
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  3. Hazel Thomson
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  4. Sjaak van der Sar
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  5. Viktor Lakics
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  6. Timothy E. Allsopp
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Editor information

Editors and Affiliations

  1. Ottawa Health Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2006 Humana Press Inc.

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Mee, P.J., O’Brien, C.M., Thomson, H., van der Sar, S., Lakics, V., Allsopp, T.E. (2006). Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 329. Humana Press. https://doi.org/10.1385/1-59745-037-5:353

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  • DOI: https://doi.org/10.1385/1-59745-037-5:353

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-498-2

  • Online ISBN: 978-1-59745-037-9

  • eBook Packages: Springer Protocols

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