Summary
The Wilms tumor gene was identified as a tumor suppressor gene responsible for a particu-lar type of kidney tumor. Several years ago, it was demonstrated that it is also overexpressed in acute and chronic leukemias. Although the exact role of this gene in the hematopoietic system is still quite completely obscure, it represents a reliable marker for the detection of the presence of leukemic cells. WT1 quantitative assessment may therefore represent a useful tool for the diagnosis and follow up of leukemia patients. In this chapter, we describe the method for the quantification of WT1 transcript by real-time polymerase chain reaction.
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References
Van Dongen J. J., Seriu T., Panzer-Grumayer E. R., et al. (1998) Prognostic value of minimal residual disease in acute lymphoblastic leukaemia in childhood. Lancet 28, 1731ā1738.
CavĆ©, H., van der Werfften, Bosch J., et al. (1998) Clinical significance of mini-mal residual disease in childhood acute lymphoblastic leukemia. European Orga-nization for Research and Treatment of Cancer-Childhood Leukemia Cooperative Group. N. Engl. J. Med. 27, 591ā598.
Nowell P. C. (1992) Chromosome abnormalities in myelodysplastic syndrome and acute myelogenous leukemia. Semin. Oncol. 19, 25ā33.
Cilloni D., Gottardi E., De Micheli D., et al. (2002) Quantitative assessment of WT1 expression by real time quantitative PCR may be a useful tool for monitoring minimal residual disease in acute leukemia patients. Leukemia 16, 2115ā2121.
Cilloni D., Messa F., Gottardi E., et al. (2003) Very significant correlation be-tween WT1 expression level and the IPSS score in patients with myelodysplastic syndromes. J. Clin. Oncol. 21, 1988ā1995.
Inoue K., Ogawa H., Sonoda Y., et al. (1997) Aberrant overexpression of the Wilms tumor gene (WT1) in human leukemia. Blood 89, 1405ā1412.
Call K. M., Glaser T., Ito C. Y., et al. (1990) Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus. Cell 60, 509ā520.
Madden S. L., Cook D. M., Morris J. F., Gashler A., Sukhatme V. P., and Rauscher F. J. III. (1991) Transcriptional repression mediated by the WT1 Wilms' tumor gene product. Science 253, 1550ā1553.
Park S., Schalling M., Bernard A., et al. (1993) The Wilms tumor gene WT1 is expressed in murine mesoderm-derived tissues and mutated in a human mesothelioma. Nat. Genet. 4, 415ā420.
Pritchard-Jones K. and Fleming S. (1991) Cell types expressing the Wilms' tumor gene (WT1) in Wilms' tumor: implications for tumor histogenesis. Oncogene 6, 2211ā2220.
Sekiya M., Adachi M., Hinoda Y., Imai K., and Yachi A. (1994) Downregulation of the Wilms' tumor gene (wt1) during myelomonocytic differentiation in HL60 cells. Blood 83, 1876ā1882.
Maurer U., Brieger J., Weidmann E., Mitrou P. S., Hoelzer D., and Bergmann L. (1997) The Wilms' tumor gene is expressed in a subset of CD34+ progenitors and downregulated early in the course of differentiation in vitro. Exp. Hematol. 25, 945ā950.
Cilloni D. and Saglio G. (2003) Usefulness of quantitative assessment of Wilms Tumor gene expression in chronic myeloid leukemia patients undergoing imatinib therapy. Semin. Hematol. 40, 37ā41.
Cilloni D., Gottardi E., Fava M., et al. (2003) Usefulness of quantitative assess-mentof WT1 gene transcript as a marker for minimal residual disease detection. Blood 15, 773ā774.
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Cilloni, D., Gottardi, E., Saglio, G. (2006). WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes. In: Iland, H., Hertzberg, M., Marlton, P. (eds) Myeloid Leukemia. Methods In Molecular Medicineā¢, vol 125. Humana Press. https://doi.org/10.1385/1-59745-017-0:199
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DOI: https://doi.org/10.1385/1-59745-017-0:199
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