Abstract
Epidermal growth factor (EGF) is a ligand for the EGF receptor, a member of the erbB family of receptor tyrosine kinases. Activation of EGF receptor by EGF or other high-affinity ligands often results in increased migration of cells in physiological and pathological situations. There are numerous approaches for evaluating cell migratory response following EGF stimulation. Both qualitative and quantitative techniques will be presented in this chapter.
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References
Ridley A. J., Schwartz M. A., Burridge K., et al. (2003) Cell Migration: Integratingsignals from front to back. Science 302, 1704–1709.
Singer A. J. and Clark R. A. F. (1999) Cutaneous wound healing. N. Engl. J.Med. 341, 738–746.
Hudson L. G. and McCawley L. J. (1998) Contributions of the epidermal growthfactor receptor to keratinocyte motility. Micros. Res. Tech. 43, 444–455.
Lauffenburger D. A. and Horwitz A. F. (1996) Cell migration: a physically integratedmolecular process. Cell 84, 359–369.
Pollard T. D. and Borisy G. G. (2003) Cellular motility driven by assembly anddisassembly of actin filaments. Cell 112, 453–465.
Jorissen R. N., Walker F., Pouliot, N., Garrett T. P. J., Ward C. W., andBurgessa A. W. (2003) Epidermal growth factor receptor: mechanisms of activationand signaling. Exp. Cell Res. 284, 31–53.
Moghal N. and Sternberg P. W. (2003) The epidermal growth factor system inCaenorhabditis elegans. Exp. Cell Res. 284, 150–159.
Shilo, B.-Z. (2003) Signaling by the Drosophila epidermal growth factor receptorpathway during development. Exp. Cell Res. 284, 140–149.
Holbro T., Civenni G., and Hynes N. E. (2003) The ErbB receptors and theirrole in cancer progression. Exp. Cell Res. 284, 99–110.
Arteaga C. L. (2003) ErbB-targeted therapeutic approaches in human cancer. Exp.Cell Res. 284, 122–133.
Albrecht-Buehler G. (1977) The phagokinetic tracks of 3T3 cells. Cell 11, 395–404.
Giaever I. and Keese C. R. (1991) Micromotion of mammalian-cells measuredelectrically. Proc. Natl. Acad. Sci. USA 88, 7896–7900.
Maheshwari G., Wiley H.S., and Lauffenburger, D.A. (2001) Autocrine epidermalgrowth factor signaling stimulates directionally persistent mammary epithelialcell migration. J. Cell Biol. 155, 1123–1128.
Ware M. F., Wells A., and Lauffenburger D. A. (1998) Epidermal growth factoralters fibroblast migration speed and directional persistence reciprocally and in amatrix-dependent manner. J. Cell Sci. 111, 2423–2432.
Mataraza J. M., Briggs M. W., Li Z., Entwistle, A, Ridley A.J., and Sacks, D.B. (2003) QGAP1 promotes cell motility and invasion. J. Biol. Chem. 278, 41,237-41,245.
Volberg T., Geiger B., Kartenbeck J., and Franke W. W. (1986) Changes inmembrane-microfilament interaction in intercellular adherens junctions upon removalof extracellular calcium ions. J. Cell Biol. 102, 1832–1842.
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© 2006 Humana Press Inc.
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Zeineldin, R., Hudson, L.G. (2006). Epithelial Cell Migration in Response to Epidermal Growth Factor. In: Patel, T.B., Bertics, P.J. (eds) Epidermal Growth Factor. Methods in Molecular Biology, vol 327. Humana Press. https://doi.org/10.1385/1-59745-012-X:147
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DOI: https://doi.org/10.1385/1-59745-012-X:147
Publisher Name: Humana Press
Print ISBN: 978-1-58829-421-0
Online ISBN: 978-1-59745-012-6
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