Abstracts
Noninvasive techniques for measurement of tissue pH can be invaluable in assessing disease extent and response to therapy in a variety of pathological conditions, such as renal acidosis and alkalosis, and cancers. We present the details of three techniques for noninvasive measurement of tissue pH: magnetic resonance spectroscopy (MRS), magnetic resonance spectroscopic imaging (MRSI), and contrast-enhanced magnetic resonance imaging (MRI). These techniques exploit the pH-sensitivity of three different molecules, 3-aminopropylphosphonate (3-APP), (±) 2-imidazole-1-yl-3-ethoxycarbonyl propionic acid (IEPA), and 1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetrakis(acetamidomethylenephosphonic acid) (Gd-DOTA-4AmP), to examine local extracellular pH in vivo. The level of detail presented will enable nonnovice users of MRS and MRI to reproduce these methodologies in their own laboratories.
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Raghunand, N. (2006). Tissue pH Measurement by Magnetic Resonance Spectroscopy and Imaging. In: Prasad, P.V. (eds) Magnetic Resonance Imaging. Methods in Molecular Medicine™, vol 124. Humana Press. https://doi.org/10.1385/1-59745-010-3:347
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DOI: https://doi.org/10.1385/1-59745-010-3:347
Publisher Name: Humana Press
Print ISBN: 978-1-58829-397-8
Online ISBN: 978-1-59745-010-2
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