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Use of Fluorescent Proteins as Reporters

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 323))

Abstract

Fluorescent proteins (FPs) have established themselves as valuable reporter proteins in plant molecular biology. Beside general background information about spectral properties, protein structure and maturation of different commonly used FPs, this chapter provides detailed protocols about cloning of suitable expression cassettes for GFP and DsRED and detection of FPs in Arabidopsis protoplasts and plants transiently or stably expressing these constructs by fluorescence microscopy.

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Author information

Authors and Affiliations

  1. Max Planck Institut fuer Zuechtungsforschung, Koeln, Germany

    Guido Jach

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  1. Guido Jach
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Editors and Affiliations

  1. Dept. of Biotechnology, Instituto Nacional de Investigacion y TecnologĂ­a Agraria y Alimentaria (INIA), Madrid, Spain

    Julio Salinas PhD

  2. Dept. de Genetica Molecular de Plantas, Centro Nacional de BiotecnologĂ­a-CSIC, Campus de Cantoblanco, Madrid, Spain

    Jose J. Sanchez-Serrano PhD

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© 2006 Humana Press Inc.

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Jach, G. (2006). Use of Fluorescent Proteins as Reporters. In: Salinas, J., Sanchez-Serrano, J.J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 323. Humana Press. https://doi.org/10.1385/1-59745-003-0:275

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  • DOI: https://doi.org/10.1385/1-59745-003-0:275

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-395-4

  • Online ISBN: 978-1-59745-003-4

  • eBook Packages: Springer Protocols

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