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Genotyping and Mapping With High-Density Oligonucleotide Arrays

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 323))

Abstract

This chapter discusses the specific details of how oligonucleotide arrays originally designed for expression analysis can be used for high-density genotyping to reveal tens of thousands of single-feature polymorphisms (SFPs). Cluster of SFPs reveal potential natural deletion polymorphisms. SFPs can be readily used for mapping mutations using bulk segregant analysis. An example is given using the Columbia and Landsberg erecta strains of Arabidopsis thaliana.

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© 2006 Humana Press Inc.

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Borevitz, J. (2006). Genotyping and Mapping With High-Density Oligonucleotide Arrays. In: Salinas, J., Sanchez-Serrano, J.J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 323. Humana Press. https://doi.org/10.1385/1-59745-003-0:137

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  • DOI: https://doi.org/10.1385/1-59745-003-0:137

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-395-4

  • Online ISBN: 978-1-59745-003-4

  • eBook Packages: Springer Protocols

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