Preparation and Quality Assessment of RNA From Cell-Specific Samples Obtained by Laser Microdissection

  • Regine Kleber
  • Julia Kehr
Part of the Methods in Molecular Biology™ book series (MIMB, volume 323)

Abstract

Laser microdissection is a powerful tool to obtain cell-specific isolates from complex tissue samples. This chapter outlines how to prepare plant material for microdissection and methods to extract and measure high-quality RNA suitable for a variety of different downstream applications.

Key Words

Single cell RNA extraction laser microdissection laser pressure catapulting tissue embedding 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Regine Kleber
    • 1
  • Julia Kehr
    • 1
  1. 1.Max-Planck-Institute of Molecular Plant PhysiologyGolmGermany

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