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β-Glucuronidase as Reporter Gene

Advantages and Limitations
  • Kye-Won Kim
  • Vincent R. Franceschi
  • Laurence B. Davin
  • Norman G. Lewis
Part of the Methods in Molecular Biology™ book series (MIMB, volume 323)

Abstract

The β-glucuronidase (GUS) gene is used extensively in plant biology studies; this analysis summarizes its advantages and limitations. With the advances in genomic sequencing and computational analyses (including bioinformatics), its application in the study of plant gene expression is now an integral component of modern day plant science. This chapter focuses on the detailed challenges of carrying out GUS studies for both qualitative and quantitative analyses, including the increasing employment of GUS from Bacillus strains, rather than E. coli; the Bacillus GUS genes encode proteins with enhanced properties, such as both increased thermostability and stability in the presence of crosslinking fixatives.

Key Words

β-glucuronidase (GUS) GUS-promoter fusions Arabidopsis Cruciferae gene expression localization thermostability fixatives 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Kye-Won Kim
    • 1
  • Vincent R. Franceschi
    • 2
  • Laurence B. Davin
    • 1
  • Norman G. Lewis
    • 1
  1. 1.Institute of Biological ChemistryWashington State UniversityPullman
  2. 2.Swammerdam Institute of Life ScienceUniversity of AmsterdamAmsterdamThe Netherlands

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