Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization

  • Azara Janmohamed
  • Ian R. Phillips
  • Elizabeth A. Shephard
Part of the Methods in Molecular Biology book series (MIMB, volume 320)

Abstract

Methods are described for the cellular localization of expression of flavin-containing monooxygenase (FMO) genes in various mouse tissues by in situ hybridization. These include the production of digoxigenin (DIG)-labeled antisense and sense RNA probes by transcription from FMO cDNA templates, the preparation of paraffin wax-embedded and cryostat tissue sections, the hybridization of RNA probes to tissue sections, and the specific detection of hybridized probes using an antibody to DIG.

Key Words

Flavin-containing monooxygenase antisense RNA probes in situ hybridization mouse digoxigenin-labeled probes gene expression 

Notes

Acknowledgment

This work was supported by a grant from the Wellcome Trust (no. 053590).

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Azara Janmohamed
    • 1
  • Ian R. Phillips
    • 2
  • Elizabeth A. Shephard
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity College LondonLondonUK
  2. 2.School of Biological Sciences, Queen MaryUniversity of LondonLondonUK

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