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Polymerase Chain Reaction in Miniaturized Systems:

Big Progress in Little Devices

  • Protocol
Microfluidic Techniques

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 321))

Abstract

A critical need exists for advanced technologies that enable genomic-based DNA analysis to be performed with significantly higher throughput and at a significantly lower cost than is attainable with current hardware. Miniaturized polymerase chain reaction systems offer an attractive platform to address these needs, combining the ability to perform rapid thermocycling with a portable device format that can be inexpensively mass produced. We review recent efforts aimed at developing these next-generation systems and discuss some of the practical considerations involved in adapting this technology for routine laboratory use.

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Authors and Affiliations

  1. Department of Chemical Engineering, Texas A⇐p;M University, College Station, TX

    Kalee D. Spitzack & Victor M. Ugaz

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  1. Kalee D. Spitzack
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  1. Department of Chemistry, Saint Louis University, St. Louis, MO

    Shelley D. Minteer

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Spitzack, K.D., Ugaz, V.M. (2006). Polymerase Chain Reaction in Miniaturized Systems:. In: Minteer, S.D. (eds) Microfluidic Techniques. Methods In Molecular Biology™, vol 321. Humana Press. https://doi.org/10.1385/1-59259-997-4:97

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  • DOI: https://doi.org/10.1385/1-59259-997-4:97

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-517-0

  • Online ISBN: 978-1-59259-997-4

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