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Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems

  • Jin-Woo Choi
Part of the Methods In Molecular Biology™ book series (MIMB, volume 321)

Abstract

Fabrication of micromachined magnetic particle separators is introduced with an emphasis on magnetic particle-based bioseparation in microfluidic systems. The most attractive aspect of the magnetic separation technique in biochemistry and biotechnology is ease of manipulation of biomolecules, which are immobilized on magnetic particles. Embedded electromagnets in the microfluidic channel are described for on-chip magnetic particle separation for lab-on-a-chip applications. An example of microfluidic systems with a magnetic particle separator and integrated microfluidic components is also presented for rapid magnetic particle-based bioseparation and immunoassay.

Key Words

Bioseparation magnetic particle microfluidic system microfabrication superparamagnetic particle 

References

  1. 1.
    Melville, D., Paul, F., and Roath, S. (1975) High gradient magnetic separation of red cells from whole blood. IEEE Trans. Magn. Mag-11, 1701.CrossRefGoogle Scholar
  2. 2.
    Whitesides, G. M., Kazlauskas, R. J., and Josephson, L. (1983) Magnetic Separations in Biotechnology. Trends Biotechnol. 1, 144–148.CrossRefGoogle Scholar
  3. 3.
    Roath, S., Smith, A., and Watson, J. H. P. (1990) High gradient magnetic separation in blood and bone marrow processing. J. Magnetism. Magn. Mater. 85, 285–289.CrossRefGoogle Scholar
  4. 4.
    Chikov, V., Kuznetsov, A., Shapiro, A., Winoto-Morbach, S., and Mueller-Ruchholtz, W. (1993) Single cell magnetophoresis and its diagnostic value. J. Magnetism. Magn. Mater. 122, 367–370.CrossRefGoogle Scholar
  5. 5.
    Miltenyi, S., Muller, W., Weichel, W., and Radbruch, A. (1990) High-gradient magnetic cell-separation with MACS. Cytometry 11, 231–238.PubMedCrossRefGoogle Scholar
  6. 6.
    Partington, K. M., Jenkinson, E. J., and Anderson, G. A. (1999) Novel method of cell separation based on dual parameter immunomagnetic cell selection. J. Immunol. Methods 223, 195–205.PubMedCrossRefGoogle Scholar
  7. 7.
    Hartig, R., Hausmann, M., and Cremer, C. (1995) Continuous focusing of biological particles by continuous immunomagnetic sorter: technique and applications. Electrophoresis 16, 789–792.PubMedCrossRefGoogle Scholar
  8. 8.
    Yu, H. (1998) Use of an immunomagnetic separation-fluorescent immunoassay (IMS-FIA) for rapid and high throughput analysis of environmental water samples. Anal. Chim. Acta 376, 77–81.CrossRefGoogle Scholar
  9. 9.
    Panphilov, A. S. and Bekish, O.-J. L. (1997) Updating of magnetosensitive microspheres from styrene technology for immunomagnetic cell separation. Immunol. Lett. 56, 462.Google Scholar
  10. 10.
    Ghassabian, S., Ehtezazi, T., Forutan, S. M., and Mortazavi, S. A. (1996) Dexamethasone-loaded magnetic albumin microspheres: preparation and in vitro release. Intl. J. Pharmaceutics 130, 49–55.CrossRefGoogle Scholar
  11. 11.
    Goodwin, S., Peterson, C., Hoh, C., and Bittner, C. (1999) Targeting and retention of magnetic targeted carriers (MTCs) enhancing intra-arterial chemotherapy. J. Magnetism Magn. Mater. 194, 132–139.CrossRefGoogle Scholar
  12. 12.
    Pulfer, S. K., Ciccotto, S. L., and Gallo, J. M. (1999) Distribution of small magnetic particles in brain tumor-bearing rats. J. Neuro-Oncol. 41, 99–105.CrossRefGoogle Scholar
  13. 13.
    Lubbe, A. S., Bergemann, C., Brock, J., and McClure, D. G. (1999) Physiological aspects in magnetic drug-targeting. J. Magnetism Magn. Mater. 194, 149–155.CrossRefGoogle Scholar
  14. 14.
    Deng, M. Q., Lam, K. M., and Cliver, D. O. (2000) Immunomagnetic separation of cryptosporidium parvum oocysts using MACS microbeads and high gradient separation columns. J. Microbiol. Methods 40, 11–17.PubMedCrossRefGoogle Scholar
  15. 15.
    Makarovskiy, A. N., Ackerley, W., Wojcik, L., et al. (1997) Application of immunomagnetic beads in combination with RT-PCR for the detection of circulating prostate cancer cells. J. Clin. Lab. Anal. 11, 346–350.PubMedCrossRefGoogle Scholar
  16. 16.
    Cudjoe, K. C., Hagtvedt, T., and Dainty, R. (1995) Immunomagnetic separation of salmonella from foods and their detection using immunomagnetic particle (IMP)-ELISA. Intl. J. Food Microbiol. 27, 11–25.CrossRefGoogle Scholar
  17. 17.
    Wright, A. P., Fitzgerald, J. J., and Colello, R. J. (1997) Rapid purification of glial cells using immunomagnetic separation. J. Neurosci. Methods 74, 37–44.PubMedCrossRefGoogle Scholar
  18. 18.
    Bosnes, M., Dggerdal, A., Rian, A., Korsnes, L., and Larsen, F. (1997) Magnetic separation in molecular biology, in Scientific and Clinical Applications of Magnetic Carriers (Hafeli, E. A., et al., eds.), Plenum, New York, pp. 311–322.Google Scholar
  19. 19.
    Choi, J.-W., Liakopoulos, T. M., and Ahn, C. H. (2001) An on-chip magnetic bead separator using spiral electromagnets with semi-encapsulated permalloy. Biosens. Bioelectron. 16, 409–416.PubMedCrossRefGoogle Scholar
  20. 20.
    Choi, J.-W., Ahn, C. H., Bhansali, S., and Henderson, H. T. (2000) A new magnetic bead-based, filterless bio-separator with planar electromagnet surfaces for integrated bio-detection systems. Sens. Actuators B 68, 34–39.CrossRefGoogle Scholar
  21. 21.
    Choi, J.-W., Oh, K. W., Thomas, J. H., et al. (2002) An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities. Lab Chip 2, 27–30.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Jin-Woo Choi
    • 1
  1. 1.Department of Electrical EngineeringLouisiana State UniversityBaton Rouge

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