Reversible, Room Temperature Bonding of Glass Devices for Microfluidics

  • Loranelle L. Lockyear
Part of the Methods In Molecular Biology™ book series (MIMB, volume 321)


This chapter describes a procedure for bonding glass microdevice substrates to their top plates by contact alone. This method results in devices that are robustly bonded but that can be separated, cleaned, and reused. For glass chips that have been used for applications involving the transport of hard particles, cells, or other biological material, reversible bonding provides a way of increasing the chip’s lifetime and utility. Glass microdevices that are cleaned following this procedure can be successfully used many times for electrophoresis or pressure-driven applications.

Key Words

Room temperature bonding reversible glass bonding glass microdevices 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Loranelle L. Lockyear
    • 1
  1. 1.Department of ChemistryBethany CollegeLindsborg

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