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Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification

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Food-Borne Pathogens

Part of the book series: Methods in Biotechnology™ ((MIBT,volume 21))

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Abstract

Viral food-borne illnesses have become very common in humans worldwide. Three viruses—noroviruses, rotavirus, and hepatitis A virus (HAV)—are implicated frequently in food-borne illness and have been ranked among the top 10 causes of food-borne disease over the past 10 years. The most common food vehicles for the transmission of enteric viruses to humans are shellfish, fruits, and vegetables. Foods may be contaminated by water tainted with untreated sewage or by contact with infected human food handlers. Virus concentrations in foods are usually low, as they are not able to multiply in situ. Therefore, the ability to detect traces of viruses in foods is essential in the development of tools for the investigation and possible prevention of viral disease outbreaks. Molecular approaches based on the amplification of viral RNA have been proposed for the specific and ultrasensitive detection of enteric viruses in foods. Nucleic acid sequence-based amplification (NASBA) is one of these molecular techniques showing great promise in viral detection. In this chapter, we describe two applications using NASBA techniques for the detection of hepatitis A virus and rotavirus.

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Acknowledgments

The authors wish to thank Dr. Stephen Davids for revision of the manuscript. Work described in this chapter was supported by the Conseil des recherches en Pêche et en agroalimentaire du Québec (CORPAQ).

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Authors and Affiliations

  1. Centre de Recherche STELA, Départment des Sciences des Aliments et de Nutrition, Université Laval, Quebec, Canada

    Julie Jean & Ismaïl Fliss

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  1. Julie Jean
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  2. Ismaïl Fliss
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Editors and Affiliations

  1. Microbiology Laboratory Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland

    Catherine C. Adley

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© 2006 Humana Press Inc.

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Jean, J., Fliss, I. (2006). Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification. In: Adley, C.C. (eds) Food-Borne Pathogens. Methods in Biotechnology™, vol 21. Humana Press. https://doi.org/10.1385/1-59259-990-7:171

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  • DOI: https://doi.org/10.1385/1-59259-990-7:171

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-465-4

  • Online ISBN: 978-1-59259-990-5

  • eBook Packages: Springer Protocols

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