Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources

PCR Analysis and Biological Activity
  • Rosabel Falcón
  • Tatiana d’Albuquerque
  • Maria das Graças de Luna
  • Angela Corrêa de Freitas-Almeida
  • Tomomasa Yano
Part of the Methods in Biotechnology™ book series (MIBT, volume 21)

Abstract

Aeromonas species are water-borne bacteria that are often found as environmental and food contaminants. They have been involved in human diarrhea disease and extraintestinal infections and are considered as emerging pathogens. These infections are probably acquired by food and water consumption, as there is a high prevalence of Aeromonas in the environment and food. From the species isolated, A. hydrophila, A. veronii biovar sobria, and A. caviae are the species most commonly implicated in human intestinal infections. The mechanism of pathogenesis is complex and not well understood. Aeromonas virulence is considered to be multifactorial. Toxins with hemolytic, cytotoxic, and enterotoxic activities have been described in many Aeromonas spp. The hemolytic activity of aeromonads is related to both hemolysin (aerA and hlyA) and cytolytic enterotoxin (aer) genes. Several virulence factors have been identified in strains isolated from a number of sources. It is possible that more than one of the genes involved in hemolytic/enterotoxic activity occur in the same strain. One rational approach to determine whether Aeromonas strains have the potential to be virulent is to detect the presence of hemolysin and enterotoxin genes by polymerase chain reaction (PCR) assays. PCR results can be compared with biological assays to assess the expression of the hemolytic and cytotoxic effects.

Key Words

Aeromonas aer gene aerA gene hlyA gene β-hemolysin cytolytic enterotoxin PCR Vero cells cytotoxicity assay hemolytic activity 

Notes

Acknowledgments

The authors thank Dr. Stephen Hyslop for correcting the English of the manuscript. This work was supported by grants from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), and FAPERJ (Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro), Brazil.

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Rosabel Falcón
    • 1
  • Tatiana d’Albuquerque
    • 2
  • Maria das Graças de Luna
    • 2
  • Angela Corrêa de Freitas-Almeida
    • 2
  • Tomomasa Yano
    • 3
  1. 1.Instituto Pedro KouriHavanaCuba
  2. 2.Faculdade de Ciências MédicasUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Departamento de Microbiologia e Imunologia, Instituto de BiologiaUniversidade Estadual de CampinasCampinas SPBrazil

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