Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands

  • Heiner Müller
  • Michael J. Soares
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 122)

Abstract

In this chapter, we describe protocols for the generation and characterization of alkaline phosphatase-ligand fusion proteins and their use as tools for the identification of specific ligand-receptor interactions.

Key Words

Alkaline phosphatase fusion protein SEAP protein tags placenta ligand-receptor interactions 

References

  1. 1.
    Berger, J., Hauber, J., Hauber, R., Geiger, R., and Cullen, B. R. (1988) Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66, 1–10.CrossRefPubMedGoogle Scholar
  2. 2.
    Brennan, C., Monschau, B., Lindberg, R., et al. (1997) Two Eph receptor tyrosine kinase ligands control axon growth and may be involved in the creation of the retinotectal map in the zebrafish. Development 124, 655–664.PubMedGoogle Scholar
  3. 3.
    Brennan, C. and Fabes, J. (2003) Alkaline phosphatase fusion proteins as affinity probes for protein localization studies. Sci STKE 2003(168), PL2.CrossRefPubMedGoogle Scholar
  4. 4.
    Cheng, H. J. and Flanagan, J. G. (1994) Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases. Cell 79, 157–168.CrossRefPubMedGoogle Scholar
  5. 5.
    Cheng, H. J., Nakamoto, M., Bergemann, A. D., and Flanagan, J. G. (1995) Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map. Cell 82, 371–381.CrossRefPubMedGoogle Scholar
  6. 6.
    Chiang, M. K. and Flanagan, J. G. (1995) Interactions between the Flk-1 receptor, vascular endothelial growth factor, and cell surface proteoglycan identified with a soluble receptor reagent. Growth Factors 12, 1–10.CrossRefPubMedGoogle Scholar
  7. 7.
    Chiang, M. K. and Flanagan, J. G. (1996) PTP NP, a new member of the receptor protein tyrosine phosphatase family, implicated in development of nervous system and pancreatic endocrine cells. Development 122, 2239–32250.PubMedGoogle Scholar
  8. 8.
    Flanagan, J. G. and Leder, P. (1990) The kit ligand: a cell surface molecule altered in steel mutant fibroblasts. Cell 63, 185–194.CrossRefPubMedGoogle Scholar
  9. 9.
    Flanagan, J. G. and Cheng, H. J. (2000) Alkaline phosphatase fusion proteins for molecular characterization and cloning of receptors and their ligands. Methods Enzymol. 327, 198–210.CrossRefPubMedGoogle Scholar
  10. 10.
    Tartaglia, L. A., Dembski, M., Weng, X., et al. (1995) Identification and expression cloning of a leptin receptor, OB-R. Cell 83, 1263–1271.CrossRefPubMedGoogle Scholar
  11. 11.
    Nagira, M., Imai, T., Hieshima, K., et al. (1997) Molecular cloning of a novel human CC chemokine secondary lymphoid-tissue chemokine that is a potent chemoattractant for lymphocytes and mapped to chromosome 9p13. J. Biol. Chem. 272, 19,518–19,524.CrossRefPubMedGoogle Scholar
  12. 12.
    Fong, A. M., Erickson, H. P., Zachariah, J. P., et al. (2000) Ultrastructure and function of the fractalkine mucin domain in CX(3)C chemokine domain presentation. J. Biol. Chem. 275, 3781–3786.CrossRefPubMedGoogle Scholar
  13. 13.
    Müller, H., Dai, G., and Soares, M. J. (1998) Placental lactogen-I (PL-I) target tissues identified with an alkaline phosphatase-PL-I fusion protein. J. Histochem. Cytochem. 46, 737–743.PubMedGoogle Scholar
  14. 14.
    Müller, H., Liu, B., Croy, B. A., et al. (1999) Uterine natural killer cells are targets for a trophoblast cell-specific cytokine, prolactin-like protein-A. Endocrinology 140, 2711–2720.CrossRefPubMedGoogle Scholar
  15. 15.
    Wang, D., Ishimura, R., Walia, D. S., et al. (2000) Eosinophils are cellular targets of the novel uteroplacental heparin-binding cytokine decidual/trophoblast prolactin-related protein. J. Endocrinol. 167, 15–28.CrossRefPubMedGoogle Scholar
  16. 16.
    Lin, J. and Linzer, D. I. (1999) Induction of megakaryocyte differentiation by a novel pregnancy-specific hormone. J. Biol. Chem. 274, 21,485–21,489.CrossRefPubMedGoogle Scholar
  17. 17.
    Zhou, B., Lum, H. E., Lin, J., and Linzer, D. I. (2002) Two placental hormones are agonists in stimulating megakaryocyte growth and differentiation. Endocrinology 143, 4281–4286.CrossRefPubMedGoogle Scholar
  18. 18.
    Noel, S., Herman, A., Johnson, G. A., et al. (2003) Ovine placental lactogen specifically binds to endometrial glands of the ovine uterus. Biol. Reprod. 68, 772–780.CrossRefPubMedGoogle Scholar
  19. 19.
    Tanaka, T., Shiu, R. P. C., Gout, P. W., Beer, C. T., Noble, R. L., and Friesen, H. G. (1980) A new sensitive and specific bioassay for lactogenic hormones: measurement of prolactin and growth hormone in human serum. J. Clin. Endocrinol. Metab. 51, 1058–1063.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Heiner Müller
    • 1
  • Michael J. Soares
    • 2
  1. 1.Department of Obstetrics and GynecologyUniversity of RostockRostockGermany
  2. 2.Institute of Maternal-Fetal Biology, Division of Cancer & Developmental Biology, Department of Pathology & Laboratory MedicineUniversity of Kansas Medical CenterKansas City

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