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In Vitro Models for Studying Trophoblast Transcellular Transport

  • Claudia J. Bode
  • Hong Jin
  • Erik Rytting
  • Peter S. Silverstein
  • Amber M. Young
  • Kenneth L. Audus
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 122)

Abstract

In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.

Key Words

Trophoblast cells BeWo cell transcellular transport efflux mechanisms 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Claudia J. Bode
    • 1
  • Hong Jin
    • 1
  • Erik Rytting
    • 1
  • Peter S. Silverstein
    • 1
  • Amber M. Young
    • 1
  • Kenneth L. Audus
    • 1
  1. 1.Department of Pharmaceutical Chemistry, School of PharmacyUniversity of KansasLawrence

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