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Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR

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DNA Repair Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 314))

Abstract

There is a need to analyze the formation of DNA lesions in specific sequence contexts. The formation and repair of DNA damage at specific locations in the genome is modulated by the DNA sequence, by DNA methylation patterns, by the transcriptional status of the locus, and by chromatin proteins associated with the DNA. The only method currently available to allow a precise sequence mapping of DNA lesions in mammalian cells is the ligation-mediated polymerase chain reaction (LM-PCR). I describe the technical details of LM-PCR as exemplified by the mapping of DNA damage products in ultraviolet (UV) light-irradiated cells.

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Acknowledgments

We thank A. Sancar for kindly providing E. coli photolyase. This work has been supported by a grant from the National Institute of Environmental Health Sciences (ES06070) to G.P.P.

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Authors and Affiliations

  1. Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA

    Gerd P. Pfeifer

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  1. Gerd P. Pfeifer
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Editors and Affiliations

  1. Pharmacological Sciences, SUNY Stony Brook, Stony Brook, NY

    Daryl S. Henderson

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© 2006 Humana Press Inc.

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Pfeifer, G.P. (2006). Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR. In: Henderson, D.S. (eds) DNA Repair Protocols. Methods in Molecular Biology™, vol 314. Humana Press. https://doi.org/10.1385/1-59259-973-7:201

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  • DOI: https://doi.org/10.1385/1-59259-973-7:201

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-513-2

  • Online ISBN: 978-1-59259-973-8

  • eBook Packages: Springer Protocols

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