Summary
The guanidinium acid-phenol method of RNA extraction is relatively fast (4 h) and is useful for the processing of large numbers of samples, without the need for ultracentrifugation. This protocol produces total RNA that includes ribosomal, transfer, and messenger RNA.
This high-quality RNA is suitable for Northern blot analysis, dot-blot hybridization, poly (A) RNA selection, in vitro translation, cDNA library construction, reverse transcriptase-polymerase chain reaction, ribonuclease protection assay, and primer extension experiments.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.
Kingston, R. E., Chomczynski, P., and Sacchi, N. (1991) Guanidinium methods for total RNA preparation, in Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kingston, R. E., et al., eds.) Wiley, New York, NY, (Suppl 14), pp. 4.2.1–4.2.8.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Humana Press Inc.
About this protocol
Cite this protocol
Robertson, N., Leek, R. (2006). Isolation of RNA From Tumor Samples. In: Brooks, S.A., Harris, A. (eds) Breast Cancer Research Protocols. Methods in Molecular Medicine, vol 120. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-969-9:55
Download citation
DOI: https://doi.org/10.1385/1-59259-969-9:55
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-191-2
Online ISBN: 978-1-59259-969-1
eBook Packages: Springer Protocols