Summary
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting to detect proteins and glycoproteins is one of the most widely used and broadly useful techniques in cancer research, allowing the proteins in a complex sample—such as a blood fsample, aspirate, or solid tumor homogenate—to be separated according to molecular weight and visualized within a gel matrix and/or, once separated, transferred onto a supporting membrane, where they may be probed for the binding of antibodies or lectins. In this chapter, the theory and principles of SDS-PAGE and Western blotting are briefly outlined, and basic methods are given that can be applied to investigate virtually any (glyco)protein of interest in breast cancer research.
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References
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Osborne, C., Brooks, S.A. (2006). SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research. In: Brooks, S.A., Harris, A. (eds) Breast Cancer Research Protocols. Methods in Molecular Medicine, vol 120. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-969-9:217
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DOI: https://doi.org/10.1385/1-59259-969-9:217
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-191-2
Online ISBN: 978-1-59259-969-1
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