Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA

  • Naomi Robertson
  • Russell Leek
Part of the Methods in Molecular Medicine book series (MIMM, volume 120)


This chapter outlines methods for the preparation of several different cellular fractions from whole samples of tumor and normal tissue.

Key Words

Cytosol membrane fraction tumor homogenate HEPES 

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Naomi Robertson
    • 1
  • Russell Leek
    • 2
  1. 1.Cancer Research UK Molecular Oncology Laboratories, Weatherall Institute of Molecular MedicineThe John Radcliffe HospitaOxfordUK
  2. 2.Cancer Research UK Tumour Pathology Group, The John Radcliffe HospitalUniversity of Oxford Medical Sciences DivisionOxfordUK

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