Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis

  • Louise E. Reynolds
  • Kairbaan M. Hodivala-Dilke
Part of the Methods in Molecular Medicine book series (MIMM, volume 120)


The growth of new blood vessels, known as angiogenesis, is a dynamic but highly regulated process involving many different regulatory pathways. Endothelial cell migration and proliferation is also essential for this process to occur. Studying the behavior of endothelial cells and how they repond to the factors involved in angiogenesis is important in understanding how the process is controlled. The ability to generatic primary endothelial cells allows for such detailed studies. In this chapter, we present the method for endothelial cell isolation that we have found to be successful and that we routinely employ to isolate and culture primary endothelial cells.

Key Words

Angiogenesis endothelial cells macrophages cell sorting cell culture 


  1. 1.
    Folkman, J. and Shing, Y. (1992) Angiogenesis. J. Biol. Chem. 267, 10,931–10,934.PubMedGoogle Scholar
  2. 2.
    Folkman, J. (1974) Tumor angiogenesis. Adv. Cancer Res. 19, 331–358.PubMedCrossRefGoogle Scholar
  3. 3.
    Cockerill, G. W., Gamble, J. R., and Vadas, M. A. (1995) Angiogenesis: models and modulators. Int. Rev. Cytol. 159, 113–160.PubMedCrossRefGoogle Scholar
  4. 4.
    Bischoff, J. (1995) Approaches to studying cell adhesion molecules in angiogenesis. Trends Cell Biol. 5, 69–74.PubMedCrossRefGoogle Scholar
  5. 5.
    Bach, T. L., Barsigian, C., Chalupowicz, D. G., et al. (1998) VE-Cadherin mediates endothelial cell capillary tube formation in fibrin and collagen gels. Exp. Cell Res. 238, 324–334.PubMedCrossRefGoogle Scholar
  6. 6.
    Reynolds, L. E., Wyder, L., Lively, J., et al. (2002) Enhanced pathological angiogenesis in β3 and β3/β5 double knockout mice. Nat. Med. 8, 27–34.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Louise E. Reynolds
    • 1
  • Kairbaan M. Hodivala-Dilke
    • 1
  1. 1.Department of Tumour Biology, Cell Adhesion and Disease Laboratory, Cancer Research UK, John Vane Science Centre, Barts and the London School of Medicine and DentistryCharterhouse SquareLondonUK

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