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Assays for Nitric Oxide Expression

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Mast Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 315))

Abstract

The purpose of this chapter is to review the analytical methodology specifically associated with studying the role of nitric oxide (NO) in mast cell physiology and biochemistry. The methodology for measuring cellular secretion of nitric oxide with Griess Reagent will be described in detail, as well as the use of 4,5-diaminofluorescein diacetate for continuous monitoring of nitric oxide production in live cells. We will point out the limitations of the analytical techniques and also indicate areas in which promising analytical techniques have not yet been applied to the study of mast cell physiology, that is, new research opportunities. In addition to reviewing the methodology associated with measuring NO itself, we will briefly touch upon some analytical methods important in characterizing the biochemical products formed from nitric oxide (e.g., 3-nitrotyrosine).

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Acknowledgments

This work was supported by the U.S. Army Medical Research and Material Command under Contract/Grant/Intergovernmental Project Order DAMD17-98164001 (to W.L. Stone).

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Authors and Affiliations

  1. Department of Pediatrics, Quillen College of Medicine, East Tennessee State University, Johnson City, TN

    William L. Stone, Hongsong Yang & Min Qui

Authors
  1. William L. Stone
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  2. Hongsong Yang
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  3. Min Qui
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Editors and Affiliations

  1. Quillen College of Medicine, James H. Quillen Veterans Affairs Medical Center, East Tennessee State University, Johnson City, TN

    Guha Krishnaswamy  & David S. Chi  & 

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© 2006 Humana Press Inc.

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Stone, W.L., Yang, H., Qui, M. (2006). Assays for Nitric Oxide Expression. In: Krishnaswamy, G., Chi, D.S. (eds) Mast Cells. Methods in Molecular Biology, vol 315. Humana Press. https://doi.org/10.1385/1-59259-967-2:245

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  • DOI: https://doi.org/10.1385/1-59259-967-2:245

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-374-9

  • Online ISBN: 978-1-59259-967-7

  • eBook Packages: Springer Protocols

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