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Expression of Cell Surface Antigens on Mast Cells

Mast Cell Phenotyping

  • Protocol
Mast Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 315))

Abstract

During the past few decades, a number of functionally important cell surface antigens have been detected on human mast cells (MCs). These antigens include the stem cell factor receptor (SCFR/CD117), the high-affinity immunoglobulin E receptor, adhesion molecules, and activation-linked membrane determinants. Several of these antigens (CD2, CD25, CD35, CD88, CD203c) appear to be upregulated on MCs in patients with systemic mastocytosis and therefore are used as diagnostic markers. Quantitative measurement of these markers on MCs is thus of diagnostic value and is usually performed by multicolor-based flow cytometry techniques utilizing a PE- or APC-labeled antibody against CD117 for MCs detection. This chapter gives an overview about the methods of staining of MC in various tissues with special reference to novel diagnostic markers applied in patients with suspected systemic mastocytosis.

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Author information

Authors and Affiliations

  1. Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria

    Alexander W. Hauswirth, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr & Peter Valent

  2. Department of Internal Medicine I, Division of Hematology and Hemostaseology, University of Vienna, Vienna, Austria

    Stefan Florian

  3. Department of Internal Medicine II, Division of Angiology, Medical University of Vienna, Vienna, Austria

    Gerit-Holger Schernthaner

Authors
  1. Alexander W. Hauswirth
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  2. Stefan Florian
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  3. Gerit-Holger Schernthaner
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  4. Maria-Theresa Krauth
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  5. Karoline Sonneck
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  6. Wolfgang R. Sperr
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  7. Peter Valent
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Editor information

Editors and Affiliations

  1. Quillen College of Medicine, James H. Quillen Veterans Affairs Medical Center, East Tennessee State University, Johnson City, TN

    Guha Krishnaswamy  & David S. Chi  & 

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© 2006 Humana Press Inc.

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Hauswirth, A.W. et al. (2006). Expression of Cell Surface Antigens on Mast Cells. In: Krishnaswamy, G., Chi, D.S. (eds) Mast Cells. Methods in Molecular Biology, vol 315. Humana Press. https://doi.org/10.1385/1-59259-967-2:077

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  • DOI: https://doi.org/10.1385/1-59259-967-2:077

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-374-9

  • Online ISBN: 978-1-59259-967-7

  • eBook Packages: Springer Protocols

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