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Mast Cells pp 363–381Cite as

In Vivo Models for Studying Mast Cell-Dependent Responses to Bacterial Infection

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Part of the Methods in Molecular Biology book series (MIMB,volume 315)

Abstract

Mast cells are a critical component of host defense against bacterial infections. Activation of these cells during infection induces both innate and adaptive aspects of protective immunity needed for the elimination of the bacteria and survival of the host. These functional roles for the mast cell have been principally characterized using two in vivo models of acute bacterial infection featuring Gram-negative pathogens such as Escherichia coli. Here, we present basic protocols for the identification of mast cell-dependent biological functions during bacterial infection. These include the use of mast cell-deficient mice, the identification of mast cells in tissue, the culture of uropathogenic E. coli, and the basic analysis of mast cell-dependent functions in the peritoneal cavity and footpad models of bacterial pathogenesis.

Key Words

  • Mast cell
  • bacteria
  • E. coli
  • UPEC
  • tumor necrosis factor
  • peritoneal cavity
  • footpad
  • draining popliteal lymph node

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  • DOI: 10.1385/1-59259-967-2:363
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Acknowledgments

This work was supported by funds from the National Institutes of Health DK50814 and AI50021 and the Sandler Foundation for Asthma Research.

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Authors and Affiliations

  1. Department of Pathology, Duke University, Durham, NC

    Christopher P. Shelburne & James B. McLachlan

  2. Department of Pathology, Molecular Genetics and Microbiology and Immunology, Duke University Medical Center, Durham, NC

    Soman N. Abraham

Authors
  1. Christopher P. Shelburne
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  2. James B. McLachlan
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  3. Soman N. Abraham
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Editor information

Editors and Affiliations

  1. Quillen College of Medicine, James H. Quillen Veterans Affairs Medical Center, East Tennessee State University, Johnson City, TN

    Guha Krishnaswamy & David S. Chi & 

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© 2006 Humana Press Inc.

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Shelburne, C.P., McLachlan, J.B., Abraham, S.N. (2006). In Vivo Models for Studying Mast Cell-Dependent Responses to Bacterial Infection. In: Krishnaswamy, G., Chi, D.S. (eds) Mast Cells. Methods in Molecular Biology, vol 315. Humana Press. https://doi.org/10.1385/1-59259-967-2:363

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  • DOI: https://doi.org/10.1385/1-59259-967-2:363

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-374-9

  • Online ISBN: 978-1-59259-967-7

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