Measurement of Mast Cell Cytokine Release by Multiplex Assay
Mast cells are highly responsive cells that are capable of secreting a variety of inflammatory mediators, including histamine, heparin, serine proteases, leukotrienes, prostaglandins, and thromboxanes. Studies from several laboratories have demonstrated that mast cells have the capacity to produce a variety of cytokines in response to various stimuli. Characterization of the cytokine profiles in mast cells has routinely been determined by the performance of individual enzyme-linked immunosorbent assays. This process is expensive, time-consuming, and requires a great deal of material to characterize multiple cytokines. In this chapter, we describe a multiplex cytokine assay to detect 17 cytokines simultaneously in 50 µL of culture supernatant derived from stimulated human cord blood-derived mast cells.
Key WordsMast cells cord blood-derived mast cells Luminex® xMAP™ Bio-Plex 17-plex cytokine assay cytokines microsphere multiplex
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