Abstract
Virus-induced gene silencing (VIGS) has been used as a quick and easy tool to assess gene function in plants. The virus, carrying a portion of an endogenous gene, triggers a homology-based defense mechanism when introduced into plants. Infection with the modified virus results in gene-specific transcript degradation. Several virus-based vectors have been developed for use in VIGS. One of these virus vectors, tobacco rattle virus (TRV), has a large host range and is transmitted by phytopathogenic nematodes of the genera Trichodorus and Paratrichodorus. TRV has been used in VIGS to study gene function in above-ground parts of plants. This chapter describes a protocol for the use of TRV vector to silence genes in roots.
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This research was supported by a grant from the University of California Agricultural Experiment Station.
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Kaloshian, I. (2007). Virus-Induced Gene Silencing in Plant Roots. In: Ronald, P.C. (eds) Plant-Pathogen Interactions. Methods in Molecular Biology, vol 354. Humana Press. https://doi.org/10.1385/1-59259-966-4:173
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DOI: https://doi.org/10.1385/1-59259-966-4:173
Publisher Name: Humana Press
Print ISBN: 978-1-58829-448-7
Online ISBN: 978-1-59259-966-0
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