Abstract
Massively parallel signature sequencing is a sequencing-based method that provides quantitative gene expression data for nearly all transcripts in a particular ribonucleic acid sample. Although the sequencing technology is practiced as a service by a California-based company, we have developed methods for the handling and analysis of these data. This chapter describes the steps involved in obtaining data from massively parallel signature sequencing, aligning the signatures to genomic sequence, identifying novel transcripts, and performing quantitative analyses of genes expressed under conditions such as disease treatments.
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Acknowledgment
The methods described here were developed with support from the National Science Foundation Plant Genome Research Program.
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Meyers, B.C., Haudenschild, C.D., Vemaraju, K. (2007). Use of Massively Parallel Signature Sequencing to Study Genes Expressed During the Plant Defense Response. In: Ronald, P.C. (eds) Plant-Pathogen Interactions. Methods in Molecular Biology, vol 354. Humana Press. https://doi.org/10.1385/1-59259-966-4:105
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DOI: https://doi.org/10.1385/1-59259-966-4:105
Publisher Name: Humana Press
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