Skip to main content

An Efficient Method for the Micropropagation of Agave Species

  • Protocol

Part of the Methods in Molecular Biology™ book series (MIMB,volume 318)

Abstract

Despite their economic importance, the Agave spp. have not been genetically improved. This is probably owing to the fact that they have very long life cycles and many of them have an inefficient sexual reproduction mechanism. Micropropagation offers an alternative to this problem through the efficient cloning of selected high-yielding “elite” plants. We report here efficient method to micropropagate agaves and a strategy for the management of large scale production that has been successfully applied to several Agave spp.

Key Words

  • Agave
  • genetic improvement
  • large-scale propagation
  • micropropagation

This is a preview of subscription content, access via your institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1385/1-59259-959-1:165
  • Chapter length: 14 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   139.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-959-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   179.00
Price excludes VAT (USA)
Hardcover Book
USD   219.99
Price excludes VAT (USA)
Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Lock, G. W. (1985) On the scientific and practical aspects of sisal (Agave sisalana) cultivation, in Biología y Aprovechamiento Integral del Henequen y Otros Agaves (Cruz, C., Del Castillo, L., Robert, M. L., and Ordanza, R. N., eds.), Centro de Investigacióm Científica de Yucatán A. C., México, pp 99–119.

    Google Scholar 

  2. Robert, M. L., Herrera, J. L., Contreras, F., and Scorer, K. N. (1987) In vitro propagation of Agave fourcroydes Lem. (Henequen). Plant Cell Tiss. Org. Cult. 8, 37–48.

    CAS  CrossRef  Google Scholar 

  3. Robert, M. L., Herrera, J. L., Chan, J. L., and Contreras, F. (1992) Micropropagation of Agave ssp. (Henequen), in Biotechnology in Agriculture and Forestry, 19 (Bajaj, Y. P. S., ed.), Springer-Verlag, Berlin Heidelberg, pp 305–329.

    Google Scholar 

  4. Barredo-Pool, F. A., Piven, M., Borges-Argaez, I, C., Herrera, J. L., Herrera, M. A. and Robert, M. L. (2004) Formación de Brotes Adventicios y Embriones Som·ticos en Explantes de Tallo de Henequén (Agave fourcroydes Le Cultivados in vitro. IV Simposio Internacional sobre Agavaceae y Nolinaceae. Mérida, Yucatán, México.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2006 Humana Press Inc.

About this protocol

Cite this protocol

Robert, M.L., Herrera-Herrera, J.L., Castillo, E., Ojeda, G., Herrera-Alamillo, M.A. (2006). An Efficient Method for the Micropropagation of Agave Species. In: Loyola-Vargas, V.M., Vázquez-Flota, F. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology™, vol 318. Humana Press. https://doi.org/10.1385/1-59259-959-1:165

Download citation

  • DOI: https://doi.org/10.1385/1-59259-959-1:165

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-547-7

  • Online ISBN: 978-1-59259-959-2

  • eBook Packages: Springer Protocols