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Measurement of Cell Viability in In Vitro Cultures

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 318)

Abstract

An overview of the methods for assessing cell viability in in vitro cultures is presented. The protocols of four of the most commonly used assays are described in detail, so the readers may be able to determine which assay is suitable for their own projects using plant cell cultures.

Key Words

  • Cell viability
  • Evans blue
  • fluorescein diacetate
  • MTT
  • TTC
  • tetrazolium salts

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  • DOI: 10.1385/1-59259-959-1:071
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© 2006 Humana Press Inc.

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Castro-Concha, L.A., Escobedo, R.M., de Miranda-Ham, M.L. (2006). Measurement of Cell Viability in In Vitro Cultures. In: Loyola-Vargas, V.M., Vázquez-Flota, F. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology™, vol 318. Humana Press. https://doi.org/10.1385/1-59259-959-1:071

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  • DOI: https://doi.org/10.1385/1-59259-959-1:071

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-547-7

  • Online ISBN: 978-1-59259-959-2

  • eBook Packages: Springer Protocols