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Callus and Suspension Culture Induction, Maintenance, and Characterization

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 318)

Abstract

Callus and cell suspension can be used for long-term cell cultures maintenance. This chapter describes procedures for the induction of somatic embryos of garlic, keeping a regeneration capacity for more than 5 yr, as well as the maintenance of a tobacco suspension culture (NT-1 cells), for more than 10 yr. Methods for plant regeneration and growth kinetics of garlic cultures are described, as well as for cell viability of NT-1 cells stained with 2,3,5 triphenyltetrazolium chloride. The packed cell volume determination as a parameter of growth is detailed.

Key Words

  • Allium sativum
  • callus cultures
  • garlic
  • somatic embryogenesis
  • suspension cultures

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  • DOI: 10.1385/1-59259-959-1:059
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References

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Acknowledgments

The authors want to thank Laura Silva-Rosales for her critical review of the manuscript and Andres Estrada and Marcelina Garcia for their technical assistance in the TTC staining and observations.

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© 2006 Humana Press Inc.

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Robledo-Paz, A., Vázquez-Sánchez, M.N., Adame-Alvarez, R.M., Jofre-Garfias, A.E. (2006). Callus and Suspension Culture Induction, Maintenance, and Characterization. In: Loyola-Vargas, V.M., Vázquez-Flota, F. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology™, vol 318. Humana Press. https://doi.org/10.1385/1-59259-959-1:059

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  • DOI: https://doi.org/10.1385/1-59259-959-1:059

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-547-7

  • Online ISBN: 978-1-59259-959-2

  • eBook Packages: Springer Protocols