Skip to main content

Growth Measurements

Estimation of Cell Division and Cell Expansion

  • Protocol

Part of the Methods in Molecular Biology™ book series (MIMB,volume 318)


The main parameters for the estimation of growth within in vitro cultures are reviewed. Procedures to measure these parameters are described, emphasizing in each case their convenience of use, depending on the features of the culture to evaluate.

Key Words

  • Cell counting
  • duplication time
  • dry weight
  • fresh weight
  • growth index
  • packed cell volume

This is a preview of subscription content, access via your institution.

Buying options

USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1385/1-59259-959-1:051
  • Chapter length: 8 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
USD   139.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-959-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   179.00
Price excludes VAT (USA)
Hardcover Book
USD   219.99
Price excludes VAT (USA)

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more


  1. Ryu, D. D. Y., Lee, S. O., and Romani, R. J. (1990) Determination of growth rate for plant cell cultures: comparative studies. Biotechnol. Bioeng. 35, 305–311.

    CAS  CrossRef  PubMed  Google Scholar 

  2. Fowler, M. W. (1987) Products from plant cells, in Basic Biotechnology (Bu’lok, J. and Kristiansen, B., eds.), Academic Press, London, pp 525–544.

    Google Scholar 

  3. Su, W. W. (1995) Bioprocessing technology for plant cell suspension cultures. Appl. Biochem. Biotechnol. 50, 189–230.

    CAS  CrossRef  Google Scholar 

  4. Fowler, M. W. (1982) The large scale cultivation of plant cells, in Progress in Industrial Microbiology, Vol 16. (Bull, M. J., ed.), Elsevier, Amsterdan, pp. 207–229.

    Google Scholar 

  5. Trejo-Tapia, G., Arias-Castro, C., and Rodríguez-Mendiola, M. (2003) Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica. Plant Cell Tiss. Org. Cult. 72, 7–12.

    CAS  CrossRef  Google Scholar 

  6. King, P. J. and Street, H. E. (1977) Growth patterns in cell cultures, in Botanical Monographs, Vol 11: Plant Tissue and Cell Culture (Street, H. E., ed.), Blackwell, Oxford.

    Google Scholar 

  7. Ramage, C. M. and Williams, R. R. (2002) Mineral nutrition and plant morphogenesis. In Vitro Cell Dev. Biol., Plant 38, 116–124.

    CAS  CrossRef  Google Scholar 

Download references

Author information

Authors and Affiliations


Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2006 Humana Press Inc.

About this protocol

Cite this protocol

Godoy-Hernández, G., Vázquez-Flota, F.A. (2006). Growth Measurements. In: Loyola-Vargas, V.M., Vázquez-Flota, F. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology™, vol 318. Humana Press.

Download citation

  • DOI:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-547-7

  • Online ISBN: 978-1-59259-959-2

  • eBook Packages: Springer Protocols