Skip to main content

An Introduction to Plant Cell Culture

Back to the Future

  • Protocol

Part of the Methods in Molecular Biology™ book series (MIMB,volume 318)

Abstract

Plant cell, tissue, and organ culture is a set of techniques designed for the growth and multiplication of cells and tissues using nutrient solutions in an aseptic and controlled environment. This technology explores conditions that promote cell division and genetic reprogramming in in vitro conditions. Mainly developed in the early 1960s, plant tissue culture has turned into a standard procedure for modern biotechnology and today one can recognize five major areas where in vitro cell cultures are currently applied: large-scale propagation of elite materials, generation of genetic modified fertile individuals, as a model system for fundamental plant cell physiology aspects, preservation of endangered species, and metabolic engineering of fine chemicals. This chapter reviews the recent advances in such areas.

Key Words

  • Aseptic culture
  • genetic modified organisms
  • large-scale propagation
  • metabolic engineering
  • plant cell culture
  • techniques

This is a preview of subscription content, access via your institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1385/1-59259-959-1:003
  • Chapter length: 6 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   139.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-959-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   179.00
Price excludes VAT (USA)
Hardcover Book
USD   219.99
Price excludes VAT (USA)
Fig. 1.

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2006 Humana Press Inc.

About this protocol

Cite this protocol

Loyola-Vargas, V.M., Vázquez-Flota, F. (2006). An Introduction to Plant Cell Culture. In: Loyola-Vargas, V.M., Vázquez-Flota, F. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology™, vol 318. Humana Press. https://doi.org/10.1385/1-59259-959-1:003

Download citation

  • DOI: https://doi.org/10.1385/1-59259-959-1:003

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-547-7

  • Online ISBN: 978-1-59259-959-2

  • eBook Packages: Springer Protocols