Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids

  • Elizabeta Hernández-Domínguez
  • Freddy Campos-Tamayo
  • Mildred Carrillo-Pech
  • Felipe Vázquez-Flota
Part of the Methods in Molecular Biology™ book series (MIMB, volume 318)


A protocol for the establishment of in vitro shoot cultures of Catharanthus roseus is described. Shoots can be maintained for more than 1 yr without evidence of tissue vitrification, disaggregation, or callus formation. Vindoline was the main alkaloid accumulated, reaching values similar to those found in leaves from field-grown plants, after a long period of culture. An induction methodology to reduce such waiting time is also presented.

Key Words

Catharanthus roseus elicitation in vitro shoot culture methyl jasmonate monoterpenoid indole alkaloids 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Elizabeta Hernández-Domínguez
    • 1
  • Freddy Campos-Tamayo
    • 1
  • Mildred Carrillo-Pech
    • 1
  • Felipe Vázquez-Flota
    • 1
  1. 1.Unidad de Bioquíimica y Biologíia Molecular de PlantasCentro de Investigacióon Cientíifica de YucatáanYucatáanMéexcico

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