Production of Cybrids in Brassicaceae Species

  • Maksym Vasylenko
  • Olga Ovcharenko
  • Yuri Gleba
  • Nikolay Kuchuk
Part of the Methods in Molecular Biology™ book series (MIMB, volume 318)

Abstract

This chapter describes a method of cytoplasm transfer within the brassicaceae family through Ca-PEG-mediated protoplast fusion. The method includes a protocol of nonmutagenic albinism induction based on spectinomycin-induced plastid ribosome deficiency (PRD). The proposed application of spectinomycin-mediated albinism allows speeding up creation of albino lines, as well as of hybrid production with substituted cytoplasm. According to described method cybrids between Orychophragmus violaceus and Brassica napus, O. violaceus and Lesquerella fendleri have been produced. Methods of further molecular analysis are also presented. The time-scale and reliability of described methods are indicated.

Key Words

Albinism Brassica sp cybrid spectinomycin genome segregation Lesquerella fendleri Orychophragmus violaceus plastid ribosome deficiency protoplast fusion somatic hybridization 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Maksym Vasylenko
    • 1
  • Olga Ovcharenko
    • 1
  • Yuri Gleba
    • 1
  • Nikolay Kuchuk
    • 1
  1. 1.Institute of Cell Biology and Genetic EngineeringKievUkraine

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