Abstract
The synthetic lethal screen is a method of isolating novel mutants whose survival is dependent on a gene of interest. Combining the colony-color assay with a synthetic lethal screen offers a means to visually detect a mutant that depends on a plasmid for survival. Screening for synthetic lethals can be achieved in four steps. First, the gene of interest must be mutated in a strain harboring the ade2 ade3/ade8 mutations and producing white colonies. A plasmid containing the ADE3/ADE8 gene and the wild-type gene of interest must then be transformed into the strain, which results in red colonies with white sectors where the plasmid has been lost. A mutagenesis is then required to introduce random mutations into the yeast genome. Any cell with a mutation that causes dependence on the gene of interest for survival must maintain the plasmid; these cells will produce solid red colonies. Finally, the mutants are transformed with a library. The mutants containing complementing DNA are no longer dependent on the plasmid carrying the gene of interest and thus the synthetic lethals are identified by their red-white sectoring phenotype. The synthetic lethal gene can be identified by isolating and sequencing plasmid DNA.
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© 2006 Humana Press Inc., Totowa, NJ
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Barbour, L., Xiao, W. (2006). Synthetic Lethal Screen. In: Xiao, W. (eds) Yeast Protocol. Methods in Molecular Biology, vol 313. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-958-3:161
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DOI: https://doi.org/10.1385/1-59259-958-3:161
Publisher Name: Humana Press, Totowa, NJ
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