Yeast Protocol pp 257-264 | Cite as

Study of Transcriptional Regulation Using a Reporter Gene Assay

  • Yu Fu
  • Wei Xiao
Part of the Methods in Molecular Biology book series (MIMB, volume 313)

Abstract

Study of gene expression can be facilitated by using a reporter gene assay. Instead of directly measuring the level of target gene mRNA, one can clone the promoter region of the gene of interest in front of a reporter gene and measure the reporter gene expression as a reflection of the expression of the gene of interest. We describe a simple lacZ-fusion system to measure the activity of the reporter gene product β-galactosidase. Different strategies of making the fusion construct and their applications are also discussed. This method is particularly useful to dissect the promoter region of the gene of interest and is also used in other experimental protocols such as the yeast two-hybrid analysis.

Keywords

Hydrolysis Agar Codon Recombination Chloroform 

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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Yu Fu
    • 1
  • Wei Xiao
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of SaskatchewanSaskatoonCanada

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