Extraction of Yeast Lipids

  • Roger Schneiter
  • Günther Daum
Part of the Methods in Molecular Biology book series (MIMB, volume 313)

Abstract

Quantitative extraction of lipids from the tissue or microorganism of choice is key to their subsequent analysis. In this chapter, we describe a simple and rapid protocol that relies on glass bead disruption in the presence of organic solvents to quantitatively extract lipids from yeast cells.

Keywords

Sugar Burning Filtration Chloroform MgCl2 

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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Roger Schneiter
    • 1
  • Günther Daum
    • 2
  1. 1.Division of BiochemistryUniversity of FribourgFribourgSwitzerland
  2. 2.Institute of BiochemistryGraz University of TechnologyAustria

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