Extraction of Yeast Lipids

  • Roger Schneiter
  • Günther Daum
Part of the Methods in Molecular Biology book series (MIMB, volume 313)


Quantitative extraction of lipids from the tissue or microorganism of choice is key to their subsequent analysis. In this chapter, we describe a simple and rapid protocol that relies on glass bead disruption in the presence of organic solvents to quantitatively extract lipids from yeast cells.


Glass Bead Lipid Extract Lipid Class Steryl Ester Quantitative Recovery 
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  1. 1.
    Folch, J., Lees, M., and Sloane-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509.PubMedGoogle Scholar
  2. 2.
    Bligh, E. G. and Dyer, W. J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917.PubMedCrossRefGoogle Scholar
  3. 3.
    Ways, P. and Hanahan, D. J. (1964) Characterization and quantification of red cell lipids in normal man. J. Lipid Res. 5, 318–328.PubMedGoogle Scholar
  4. 4.
    Nelson, G. J. (1975) Isolation and purification of lipids from animal tissues, in Analysis of Lipids and Lipoproteins (Perkins, E. G., ed.), Am. Oil Chem. Soc., Champaign, IL, pp. 1–22.Google Scholar
  5. 5.
    Hanson, B. A. and Lester, R. L. (1980) The extraction of inositol-containing phospholipids and phosphatidylcholine from Saccharomyces cerevisiae and Neurospora crassa. J. Lipid Res. 21, 309–315.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Roger Schneiter
    • 1
  • Günther Daum
    • 2
  1. 1.Division of BiochemistryUniversity of FribourgFribourgSwitzerland
  2. 2.Institute of BiochemistryGraz University of TechnologyAustria

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