Summary
Despite the remarkable advancement in purification methods, the isolation of small water-soluble molecules still remains a mystery for natural-product researchers. A general approach to extract and fractionate water-soluble compounds from biological materials and fluids including seawater is presented in this chapter. Some important techniques and special caution needed to deal with water-soluble compounds are also included. The chapter also presents information and discussions about various matrices, such as C18 silica gel, resins, and size-exclusion materials with respect to extraction and desalting of water-soluble compounds. In particular, applications and limitations of the increasingly popular nonionic resins are added in this edition. Four typical examples of isolation of water-soluble compounds, which were carried out in the authors’ laboratory, are presented in detail. They are: (1) the isolation of amesic shellfish poison, domoic acid, from cultured diatom cells, (2) the isolation of tetrodotoxin derivatives from a newt, (3) the isolation of an aminotetrasaccharide from cultured cyanobacterium cells, and (4) isolation of glycosidic polyether antitumor compounds from the culture broth of a marine dinoflagellate.
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Shimizu, Y., Li, B. (2006). Purification of Water-Soluble Natural Products. In: Sarker, S.D., Latif, Z., Gray, A.I. (eds) Natural Products Isolation. Methods in Biotechnology, vol 20. Humana Press. https://doi.org/10.1385/1-59259-955-9:415
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DOI: https://doi.org/10.1385/1-59259-955-9:415
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